Selective Label-Free Electrochemical Aptasensor Based on Carbon Nanotubes for Carbendazim Detection

One of the most widely used pesticides in Chile is carbendazim (CBZ), which agriculture to protect crops from fungal diseases that commonly occur rice, vegetable, and fruit crops. However, prolonged exposure it, its high persistence, can cause adverse health effects. Therefore, it necessary determine presence CBZ through rapid detection methods food samples prevent ingestion this pesticide at risk concentrations. In work, a label-free electrochemical aptasensor based on functionalized carbon nanotubes was prepared for detection. The carbodiimide reaction between amino-terminated aptamer carboxylic groups achieved covalent immobilization aptamer. immobilized changed conformation when detected blocked access redox mediator electrode surface, resulting measurable decrease voltammetric response. Under optimal conditions, featured linear range 1.0 50.0 nM, with limit 4.35 nM. Moreover, exhibited good selectivity CBZ, among other pesticides, repeatability. For tomatoes, accurately measured content sample using standard addition method. This work provides simple, rapid, sensitive, selective biosensor quantification samples.